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Unravelling the secrets of Bifidobacteria across three generations

 

New Genome Study of a Bifidobacterium subspecies by Morinaga Milk and APC Microbiome Ireland May Explain its Wide Distribution Across All Ages

 

Bifidobacterium longum is one of only a handful of species of gut bacteria that is widely present among people of all ages, including infants, adults and the elderly.1,2 Today, Morinaga Milk Industry Co., Ltd., a leading Japanese dairy product company, and the SFI Research Centre APC Microbiome Ireland, announced the results of a groundbreaking new genome study that may explain why.

Pictured Dr. Toshitaka Odamaki and Prof Douwe van Sinderen

The human gut is colonized by a collection of microbes (microbiome) which play an integral role in human health, by helping us digest our food, produce certain vitamins, regulate our immune system, and protect us against pathogenic bacteria. This current study revealed that one bacterialspecies, B. longum subsp. longum, demonstrates a wide variation in its genes that increases its competitiveness in the gut environment, because its genome adapts to the changing diet of each generation. In addition, it suggests that B. longum subsp. longum is extensively transmitted between relatives, and that such transmission does not occur only between mothers and infants, as previously believed, but also between other family members and even across three generations.3

 

For the study, a total of 453 fecal samples were collected from healthy Japanese subjects aged 0 to 104 years, as a means of measuring the bacterial species present in the gut. In total, 871 different bacterial species were detected (Fig.1) but only three species (Blautia wexlerae,Streptococcus salivarius and Bifidobacterium longum) were found to be present in more than 50% of the subjects across all generations.

 

(Fig.1) Distribution of gut microbiota in healthy Japanese subjects

 

This finding highlights the broad distribution of B. longum across the human lifespan. In order to identify the mechanism that allows a specific species to be so ubiquitous, Morinaga, in collaboration with the SFI Research Centre APC Microbiome Ireland at University College Cork, performed a comparative genome analysis on various strains of B. longum subsp. longum to determine genetic differences among strains isolated from people of varying ages.

 

B. longum subsp. longum Strains Separated into Seven Clusters

Comparative analyses involving 145 B. longum representatives obtained from Japanese subjects, including 32 publicly available genomes, showed that B. longum subsp. longum strains can be grouped into seven clusters depending on the presence of prophage (i.e. viral material) and/or a megaplasmid (i.e. genetic material being present outside of the bacterial chromosome).

 

No correlation was observed between isolated strains and subject age, although a significant negative correlation was found between the number of identified genes present in the strains and subject age. This suggests that B. longum subsp. longum strains isolated from younger subjects possess, on average, a higher number of genes compared to the ones from older subjects. Researchers performed further investigation to identify genes in each strain isolated from different age groups, and as a result, gene families were classified into four groups: infant, adult, elderly-predominant gene families, and genes that exhibit no relationship with subjects age (Fig.2).

 

(Fig.2) Genes enriched in B. longum subsp. longum strains isolated from subjects of varying age

 

Infant-associated strains more adapted to use carbohydrates found in human breast milk

 

Researchers frequently found strains of B. longum subsp. longum in younger subjects, which possess genetic code that is related to sugar utilization, including that of the sugar sialic acid which is one of the components of Human Milk Oligosaccharides (HMOs) that are found in breast milk. This characteristic may be an adaptive strategy of B. longum to survive the infant gut environment by utilizing components from the mother’s breast milk.

 

Elderly-associated strains more adapted to use insoluble fibre found in vegetables and respond to stress

 

Researchers also observed a high number of strains of B. longum subsp. longum isolated from elderly subjects that contain unique genetic information thought to encode enzymes are able to break down complex sugars that are present in plant material, such as vegetables and cereals, all of which contain a high proportion of insoluble fiber. Elderly Japanese people are reported to eat much more vegetables than younger people4, so this genetic trait to digest fiber-containing foods may allow B. longum to persist in the elderly gut.

 

In addition, two clusters of genes indicative of how bacteria respond to environmental stress were found in higher numbers among strains of B. longum subsp. longum isolated from elderly subjects, including the heat shock protein HSP20.5 Heat shock proteins are produced by organisms to help them respond to stress, such as extreme heat, oxygen exposure and starvation. It is reasonable to assume that strains with the ability to respond to stress are better able to adapt and survive.

 

Certain strains have been transmitted between father and child, husband and wife, and even across three generations

 

Several B. longum subsp. longum strains, which had been isolated from many different members of the same family, were shown to contain an essentially identical genetic content. This finding suggests that these strains had been transmitted between various family members, not only between mother and child, which has been previously reported.6

 

“It is a remarkable finding that not only was transmission between mother and child observed, but also between father and child and even between husband and wife,” he added. “This is also the first report of a particular strain of gut microbiota that appears to have been transmitted across three generations in a family, for instance, between a grandmother, mother, and infant”, stated Dr. Toshitaka Odamaki, Manager of Microbiota Research Dept. at Next Generation Science Institute of Morinaga and lead researcher of the study.

 

“We assume this variety of genes and high transfer level of the bacterial species within families is the survival strategy of B. longum subsp.longum and is key to its wide distribution across the human lifespan,” explained Dr. Odamaki. “This report could provide a promising foundation for future research aimed at identifying the best probiotic candidates at each key life stage,” he continued.

 

APC Microbiome Ireland is very interested in how the human microbiota changes over the life stages, in response to exposure to different environmental factors such as habitual diet, antibiotic exposure, and levels of stress”, said Prof Douwe van Sinderen, project leader at APC Microbiome Ireland. He furthermore emphasized the attraction of such a joint scientific effort: “This was a particularly fruitful and gratifyingcollaborative project with an industry partner, Morinaga, because we were able to join our complementary skills and know-how allowing us to generate insights that we would otherwise not have been able to obtain on our own”.

 

These findings have recently been published in the journal Scientific Reports.

 

Full reference:

Genomic diversity and distribution of Bifidobacterium longum subsp. Longum across the human lifespan

Scientific Reports 2018; 8: 85 doi   10.1038/s41598-017-18391-x; T. Odamaki , F Bottacini, K. Kato, K.Yoshida, A. Horigome, J.z.Xiao and D.van Sinderen

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758520/

 

References

  1. Turroni et al., Applied and environmental microbiology (2009)
  2. Francoise Gavini, Microb. Ecol. Health Dis (2001)
  3. Odamaki et al., Scientific Reports (2018)
  4. National Health and Nutrition Survey 2015
  5. Ventura et al., Appl. Environ. Microbiol (2007)
  6. Makino et al., Appl. Environ. Microbiol (2011)